Amnish Verma
Ministry of AYUSH, India
Title: Evaluation of anti-inflammatory activity of ‘Trikatu’ (Ayurvedic Formulation) - An experimental study
Biography
Biography: Amnish Verma
Abstract
The Anti inflammatory activity of Panchkatu, an Ayurvedic formulation, was performed on albino rats of Carrageenan induced model. 20 healthy albino rats were selected randomly and divided in to five groups , each group containing four rats were administered orally at the dosage levels as per table below : -
|
Sl.No. |
Group |
Drug |
Doses |
|
1. |
Group I (Control) |
Saline water |
1ml/100gmBody wt. |
|
2. |
Group II (Standard) |
Ibuprofen soln. |
100mg/KgBody wt. |
|
3 |
Group III (Trial) |
Panchkatu |
125mg/KgBody wt |
|
4 |
Group IV (Trial) |
Panchkatu |
250mg/KgBody wt |
|
5 |
Group V (Trial) |
Panchkatu |
500mg/KgBody wt |
The inflammatory reaction is readily produced in rats in the form of paw oedema with the help of irritants. Carrageenan induced paw oedema is most commonly used method in experimental pharmacology. The rats of treated Group II, III, IV and V were administered orally with Ibuprofen soln, Panchkatu 125mg/KgBody wt, Panchkatu 250mg/KgBody wt, Panchkatu 500mg/KgBody wt, respectively one hour before injecting 1% w/v suspension of carrageenan (0.1 ml) into the sub plantar region of left hind paw of all the five groups. Paw volume of all 20 rats were measured soon after injecting carrageenan. The volume was again measured after 1, 2, 3, 4 & 24 hrs in all the five groups of rats. The change in paw volume of Group I was compared with Group II, III, IV & V Group. Also the treated Group II, III, IV, V were also compared in between and expressed as percentage oedema inhibition by the drug.
Results of the present study are based on the oedema of hind paw of rats of all five groups measured after 1hr, 2hrs, 3hrs, 4hrs and 24hrs after carrageenan injection.
After one hr of carrageenin Inj Both Trial group Panchkatu 125mg/Kg and Panchkatu 250mg/Kg have equal percent inhibition of oedema as with Standard group (Ibuprofen) that is 42.2% while last group (Panchkatu 500mg/kg) has higher percent inhibition of oedema(50 %) than standard (Ibuprofen) (42.2%).
After two Hrs of carrageenin inj there is increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has 27.19% , Panchkatu 250 mg/Kg has 31.49% and Panchkatu 500mg/kg has 56.69% inhibition higher than Standard (Ibuprofen) 47.24%.
After three hrs of Carrageenin inj there is increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has 20% , Panchkatu 250 mg/Kg has 21.42% and Panchkatu 500mg/kg has 41.42% inhibition higher than Standard (Ibuprofen) 39.28%.
After four hrs of Carrageenin inj there is again increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has25.38% , Panchkatu 250 mg/Kg has 29.22% and Panchkatu 500mg/kg has 40.76% inhibition higher than Standard (Ibuprofen) 38.46%.
After twenty four hrs of carrageenin inj there is again increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has 3.22%,Panchkatu 250 mg/Kg has 19.35% and Panchkatu 500mg/kg has 27.41% inhibition again higher than Standard (Ibuprofen) 3.22%.
The maximum activity of all trial Groups were observed during first and second hr. and the results are significant ( P <0.005) and are comparable to standard Ibuprofen. Highest percentage oedema Inhibition was seen after 1 & 2 Hrs. Summarizing the above it is concluded that Panchkatu has showed its extreme utility or significance on the inflammation probably because of its excellent activity of inhibiting the both early released and late released mediators which is rarely seen in any anti inflammatory formulation